Identi¢cationof Bilophila wadsworthia byspeci¢cPCRwhich targets the taurine:pyruvateaminotransferase gene
نویسندگان
چکیده
targets the taurine:pyruvateaminotransferase gene Heike Laue, Theo H. M. Smits, Ulrike K. Schumacher, Marina C. Claros, Ralf Hartemink & Alasdair M. Cook Department of Biological Sciences, University of Konstanz, Konstanz, Germany; Institute for Medical Microbiology & Hygiene, EberhardKarls-University, Tübingen, Germany; Institute of Medical Microbiology and Infectious Epidemiology, University of Leipzig, Leipzig, Germany; and Department of Food Technology and Nutritional Sciences, Wageningen University and Research Centre, Wageningen, The Netherlands
منابع مشابه
Two intriguing Bilophila wadsworthia cases from Hungary.
Bilophila wadsworthia, an obligately anaerobic Gram-negative bacillus, was first isolated from appendicitis specimens and human faeces. The two cases described here are the first report of the isolation of B. wadsworthia in Hungary, and include the first isolation from chronic mastoiditis complicated with a brain abscess.
متن کاملPreliminary study using species-specific oligonucleotide probe for rRNA of Bilophila wadsworthia.
Portions of the 16S RNA from a urease-positive Bilophila wadsworthia strain were sequenced, and a probe was constructed. The probe was end labeled with [32P]ATP and polynucleotide kinase and hybridized on a nylon filter (by dot blot hybridization) to the immobilized rRNA of 12 B. wadsworthia strains and eight other anaerobic isolates. The probe efficiently hybridized only to the Bilophila strai...
متن کاملAntimicrobial susceptibility testing of Bilophila wadsworthia isolates submitted for routine laboratory examination.
MICs of antibiotics against Bilophila wadsworthia isolates were measured by agar and broth microdilution with pyruvic acid and by Etest. The inoculum size influenced greatly agar dilution. Despite discrepancies in MICs depending on the measurement method used, clindamycin consistently showed potent activity. Broth microdilution and Etest appear to be candidates for laboratory susceptibility tes...
متن کاملBilophila wadsworthia clinical isolates compared by polymerase chain reaction fingerprinting.
Bilophila wadsworthia isolates recovered from a right-ear cholesteatoma and brain abscess of the same patient were analyzed by means of polymerase chain reaction (PCR) fingerprinting with single primers (T3B and M13 core) to ascertain if they originated from the same clone. Their PCR fingerprint profiles were compared with those of three additional B. wadsworthia clinical isolates and the type ...
متن کاملColonic infection by Bilophila wadsworthia in pigs.
Bilophila wadsworthia is a common inhabitant of the human colon and has been associated with appendicitis and other local sites of inflammation in humans. Challenge-exposure or prevalence studies in laboratory and other animals have not been reported. B. wadsworthia is closely related phylogenetically to Desulfovibrio sp. and Lawsonia intracellularis, which are considered colon pathogens. We de...
متن کامل